Education
- Research Associate,
Columbia University, College of Physicians and Surgeons, 1985-1987.
- Postdoctoral Fellow, University of Chicago, Pharmacological
and Physiological Sciences, 1983-1985.
- Ph.D., Physiology, University of Illinois at Urbana-Champaign, 1980-1983.
- M.S., Physiology, University of Illinois at Urbana-Champaign, 1978-1980.
- B.S., Zoology and B.S., Environmental Biology, Eastern Illinois University, 1974-1978.
Research Interests
Investigations in my laboratory focus on understanding mechanisms and regulation of electrolyte transport across epithelial tissues and on innate immune function of the airway epithelium in relation to asthma and allergic airway disease. Cultured human airway epithelial cells and human mammary epithelial cells are currently used to study regulation of ion transport and cell migration by a variety of signaling molecules. A combination of electrophysiological approaches and molecular techniques provide a means of identifying transport mechanisms and signaling pathways that are important in controlling salt and fluid movement across these epithelia. Studies of innate immune function are being conducted in collaboration with Dr. Hirohito Kita from the Mayo Clinic. These studies address the role of the airway epithelium in allergic inflammation induced by fungal infection of the lung.
Research Projects and Grant Support
- CFTR regulation of airway epithelial cell migration
Grant support: NIH R21 HL095811
The goal of this project is to determine the role of Cystic Fibrosis Transmembrane conductance Regulator (CFTR) in the process of cell migration and wound healing in airway epithelial cells.
- Allergen-induced IL-33 release by the airway epithelium
Grant support: NIH R01 HL110539
The objective of this project is to understand the mechanism of Alternaria-induced IL-33 release from human airway epithelial cells and to explore the contribution of this important danger signal to allergic airway disease.
- Regulation of Na+ and K+ transport by the mammary epithelium
Grant support: AES-P82
The goal of this project is to examine the mechanisms of Na+ absorption and K+ secretion across human mammary epithelial cells and how transport function is regulated in response to changes in fluid movement through the ductal network.
Courses and Activities
-
Cell Physiology ANSC/PHSL 5700; BIOL 4700
- Mechanisms of Hormone Action ANSC/CMB 8344
Honors and Awards
- Teaching Excellence Award: Veterinary Physiology (CVM 6130), College of Veterinary Medicine, University of Minnesota.
- University of Minnesota Star Faculty Award for outstanding performance in research, teaching and outreach.
- Teaching Incentive Award, College of Veterinary Medicine, University of Minnesota.
- Gordon L. Starr Outstanding Contribution Award, University of Minnesota.
Professional Service
- Editorial Board Member, American Journal of Physiology, Cell Physiology
- Ad hoc NIH study section member: LCMI
Current Students
- Elizabeth R. Peitzman: Animal Physiology Graduate Program
Former Students
- Katherine R, Schiller, M.S. Ph.D. received; May 2010.
- Tamene Melkamu, DVM: Ph.D. received; April 2010.
- Melissa L. Palmer, BS: Ph.D. received; April 2005.
- So Yeong Lee, DVM: Ph.D. received; December 2002.
- Xinpo Jiang, MS: Ph.D. received; October 1999.
- Chatsri Deachapunya, MS: Ph.D. received; January 1998.
- Alisen E. Vetter, DVM: Ph.D. received; November, 1996.
- Timothy R. Traynor, BS: Ph.D. received; July, 1995.
- Leslie K Sprunger, DVM: Ph.D. received; August, 1995.
- Michael D. DuVall, DVM: Ph.D. received; June 1993.
- Gussie J. Tessier, DVM: Ph.D. received; June 1991.
Publications (2007-2011)
- Refereed Articles -
Kouzaki, H., K. Iijima, T. Kobayashi, S.M. O’Grady, and H. Kita. 2011. The danger signal, extracellular ATP, is a sensor for an airborne allergen and triggers IL-33 release and innate Th2-type responses. J. Immunol. 186:4375-4387.
Palmer, M.L., E.R. Peitzman, P.J. Maniak, G.C. Sieck, Y.S. Prakash, and S.M. O'Grady. 2011. KCa3.1 channels facilitate K+ secretion or ENaC-dependent Na+ absorption depending on apical or basolateral P2Y receptor stimulation. J. Physiol., May 23, [Epub ahead of print].
Scheckenbach, K.L., D. Losa, T. Dudez, M. Bacchetta, S.M. O'Grady, S. Crespin, and M. Chanson. 2011. PGE2 regulation of CFTR activity and air surface liquid volume requires gap junctional communication. Am. J. Respir. Cell Mol. Biol. 44:74-82.
Carlson, D.F., A.M. Geurts, J.R. Garbe, C.W. Park, A. Rangel-Filho, S.M. O'Grady, H.J. Jacob, C.J. Steer, D.A. Largaespada, and S.C. Fahrenkrug. 2010. Efficient mammalian germline transgenesis by cis-enhanced Sleeping Beauty transposition. Transgenic Res., 20:29-45.
Schiller, K.R., P.J. Maniak, and S.M. O'Grady. 2010. Cystic fibrosis transmembrane conductance regulator is involved in airway epithelial wound repair. Am. J. Physiol. [Cell Physiol.] 299:C912-921.
Bankers-Fulbright, J.L., K.R. Bartemes, G.M. Kephart, H. Kita, and S.M. O’Grady. 2009. β2 integrin-mediated adhesion and intracellular Ca2+ release in human eosinophils. J. Memb. Biol. 228:99-109.
Jang, S.H., C. Choi, S.G. Hong, O.V. Yarishkin, Y.M. Bae, J.G. Kim, S.M. O'Grady, K.A. Yoon, K.S. Kang, P.D. Ryu, and S.Y. Lee. 2009. Silencing of Kv4.1 potassium channels inhibits cell proliferation of tumorigenic human mammary epithelial cells. Biochem. Biophys. Res. Commun. 384:180-186.
Kouzaki, H., S.M. O'Grady, C.B. Lawrence, and H. Kita. 2009. Proteases induce production of thymic stromal lymphopoietin by airway epithelial cells through protease-activated receptor-2. J. Immunol. 183:1427-1434.
Melkamu, T., D. Squillace, H. Kita, and S.M. O’Grady. 2009. Regulation of TLR2 expression and function in human airway epithelial cells. J. Memb. Biol. 229:101-113.
Palmer, M.L., K.R. Schiller, and S.M. O’Grady. 2008. Apical SK potassium channels and Ca2+ dependent anion secretion in endometrial epithelial cells. J. Physiol. 586:717-726.
Lee S.Y., M.L. Palmer, P.J. Maniak, S.H. Jang, P.D. Ryu, and S.M. O'Grady. 2007. P2Y receptor regulation of sodium transport in human mammary epithelial cells. Am. J. Physiol. [Cell physiol.] 293:C1472-C1480.
- Chapters in Books -
Brown, D.R. and S.M. O'Grady. 2008. The Ussing chamber and measurement of drug actions on mucosal ion transport. In: Current Protocols in Pharmacology (John Wiley, N.Y.), 41:7.12.1-7.12.17, 2008.
- Invited Reviews -
Ingbar, D.H., M. Bhargara, and S.M. O’Grady. 2009. Mechanisms of alveolar epithelial chloride absorption. Am. J. Physiology [Lung Cell and Mol. Physiol.] 297: L813-L815.
Chanson, M., B.A. Kotsias, C. Peracchia, and S.M. O’Grady. 2007. Interaction of gap junction channels with other membrane channels and transporters. Prog. Biophys. Mol. Biol. 94:233-244.
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