Education
- Research Associate,
Columbia University, College of Physicians and Surgeons, 1985-1987.
- Postdoctoral Fellow, University of Chicago, Pharmacological
and Physiological Sciences, 1983-1985.
- Ph.D., Physiology, University of Illinois, 1980-1983.
- M.S., Physiology, University of Illinois, 1978-1980.
- B.S., Zoology, Environmental Biology, Eastern Illinois University, 1974-1978.
Research Interests
Investigations in my laboratory have focused on understanding mechanisms and regulation of electrolyte transport across epithelial tissues and regulation of eosinophil activation in patients with asthma. Cultured human airway epithelial cells and endometrial epithelial cells are currently used to study regulation of ion transport function and cell migration by a variety of signaling molecules. A combination of electrophysiological approaches and molecular techniques provide a means to identify transport mechanisms and signaling pathways that are important in controlling electrolyte and fluid transport across these epithelia. Studies of eosinophil activation conducted in collaboration with Dr. Hirohito Kita from the Mayo Clinic address the mechanism of granule acidification required for granule protein dissolution and the signaling pathways that regulate this process.
Image above: PAR-2 regulation of anion secretion across human airway epithelial cells.
Image at left: Human eosinophil exhibiting granule acidification after stimulation with platelet activating factor.
Courses and Activities
- CVM 6130: Veterinary Physiology (course coordinator)
- PHSL 8310: Advanced Topics in Cellular Physiology
- ANSC/CMB 8344: Mechanisms of Hormone Action (guest lecturer)
Honors and Awards
- Teaching Excellence Award: Veterinary Physiology (CVM 6130), College of Veterinary Medicine, University of Minnesota, 2007
- University of Minnesota Star Faculty Award for outstanding performance in research, teaching and outreach, 2005, 2006, 2007
- Teaching Incentive Awards for 1999, 2000 and 2001, College of Veterinary Medicine, University of Minnesota
- Gordon L. Starr Outstanding Contribution Award, University of Minnesota, 1992
Professional Service
- Editorial Board Member, American Journal of Physiology, Cell Physiology
- Councelor, Cell and Molecular Section, American Physiological Society
Current Students
- Tamene Melkamu, DVM: Comparative Molecular Biosciences Graduate Program
- Katie R. Schiller, M.S.: Comparative Molecular Biosciences Graduate Program
Former Students
Melissa L. Palmer, B.S.
January 2001; Ph.D. received: April 2005
Purinergic receptor regulation of ion transport in porcine endometrial gland epithelial cells
So Yeong Lee, DVM
January 1998; Ph.D. received: December 2002
P2Y receptor regulation of ion channel function in Xenopus oocytes
Xinpo Jiang, B.S.
July, 1994; Ph.D. received: October 1999
Thesis topic: Adrenergic regulation of ion transport across alveolar epithelium
Chatsri Deachapunya, M.S.
July, 1993; Ph.D. received: January 1998
Thesis topic: Regulation of transport phenotype in endometrial epithelial cells
Alisen E. Vetter, DVM
September, 1991; Ph.D. received: November 1996
Thesis topic: Regulation of electrolyte transport across the porcine endometrium
Timothy R. Traynor, B.S.
September, 1991; Ph.D. received: July 1995
Thesis topic: Functional regulation of porcine distal colon by gastrin releasing peptide
Leslie K Sprunger, DVM
July, 1989; Ph.D. received: August 1995
Thesis topic: Structure/function studies of voltage-gated K channels
Michael D. DuVall, DVM
July, 1988; Ph.D. received: June 1993
Thesis topic: Regulation of ion transport across the porcine gallbladder epithelium
Gussie J. Tessier, DVM
January, 1988; Ph.D. received: June 1991
Thesis topic: Mechanisms and regulation of electrolyte transport in equine airway epithelia
Publications (2003-2007)
- Refereed Articles -
Lee S.Y., M.L. Palmer, P.J. Maniak, S.H. Jang, P.D. Ryu, and S.M. O'Grady. 2007. P2Y receptor regulation of sodium transport in human mammary epithelial cells. Am. J. Physiol. [Cell Physiology] 293:C1472-C1480.
Palmer, M.L., S.Y. Lee, D. Carlson, S.C. Fahrenkrug, and S.M. O'Grady. 2006. Stable knockdown of CFTR establishes a role for the channel in P2Y receptor-stimulated anion secretion. J. Cell Physiol. 206:759-770.
Palmer, M.L., S.Y. Lee, P.J. Maniak, D. Carlson, S.C. Fahrenkrug, and S.M. O'Grady. 2006. Protease-activated receptor regulation of Cl- secretion in Calu-3 cells requires prostaglandin release and CFTR activation. Am. J. Physiol. [Cell Physiology]. 290:1189-1198.
O’Grady, S.M. and P.J. Maniak. 2005. Potassium secretion in winter flounder (Pseudopleuronectes americanus) intestine: Effects of K+ channel blockers on electrogenic NaCl absorption. MDIBL Bulletin 44:1-4.
Vetter, A.E. and S.M. O’Grady. 2005. Sodium and anion transport across the avian uterine (shell gland) epithelium. J. Exp. Biol. 208:479-486.
Bankers-Fulbright, J.L., G. Kephart, K.R. Bartemes, H. Kita, and S.M. O’Grady. 2004. Platelet activating factor stimulates cytoplasmic alkalinization and granule acidification in human eosinophils. J. Cell Sci. 117:5749-5757.
Lee, S.Y., R.A. Nicholas, and S.M. O’Grady. 2004. P2Y1 receptor modulation of ion channel function in Xenopus oocytes: Involvement of transmembrane domains. Purinergic Signaling 1:75-81.
Bankers-Fulbright,
J.L., G.J. Gleich, G. Kephart, H. Kita, and S.M. O'Grady. 2003.
Regulation of membrane depolarization during NADPH oxidase activation
in human eosinophils. J. Cell Sci. 116(15):3221-3226.
Boland,
L.M., M. Jiang, S.Y. Lee, S.C. Fahrenkrug, M.C. Harnett, and
S.M. O'Grady. 2003. Functional properties of a brain-specific
N-terminally spliced modulator of Kv4 channels. Am. J. Physiol.
[Cell Physiology] 285:C161-C171.
Green, B.T.,
A. Calvin, S.M. O'Grady, and D.R. Brown. 2003. Opioid and cannabinoid
inhibition of kinin-induced neurogenic anion-dependent secretion
in porcine ileum. J. Pharm. Exp. Ther. 305:733-739.
Lee, S.Y.,
P.J. Maniak, D.H. Ingbar, and S.M. O'Grady. 2003. Adult alveolar
epithelial cells express multiple subtypes of voltage-gated
K+ (Kv) channels that are located in the apical membrane. Am.
J. Physiol. [Cell physiology] 284:C1614-C1624.
Lee, S.Y.,
P.J. Maniak, R. Rhoads, D.H. Ingbar, and S.M. O'Grady. 2003.
Basolateral Cl- transport is stimulated by terbutaline in adult
rat alveolar epithelial cells. J. Memb. Biol. 191:133-139.
Lee, S.Y.,
S. Wolff, R.A. Nicholas, and S.M. O'Grady. 2003. P2Y receptors
modulate ion channel function through interactions involving
their C-terminal domains. Mol. Pharm. 63(4):878-885.
- Invited Reviews -
Chanson, M., B.A. Kotsias, C. Peracchia, and S.M. O’Grady. 2007. Interaction of gap junction channels with other membrane channels and transporters. Prog. Biophys. Mol. Biol. 94:233-244.
O’Grady, S.M. 2006. Ion transport: Potassium channels. Encyclopedia of Respiratory Medicine 8:477-482.
Palmer, M.L., A.C. Fahrenkrug, and S.M. O’Grady. 2006. RNA interference and ion channel physiology. Cell Biochem. Biophys. 46:1-17.
O’Grady, S.M. and S.Y. Lee. 2005. Molecular diversity and function of voltage-gated (Kv) potassium channels in epithelial cells. Int. J. Biochem. Cell Biol. 37:1578-1594.
Lee, S.Y. and S.M. O’Grady. 2003. Modulation of ion channel function by P2Y receptors. Cell Biochem. Biophys. 39:75-88.
O'Grady,
S.M. and S.Y. Lee. 2003. Chloride and potassium channels in
alveolar epithelial cells. Am. J. Physiology (Lung Cell and
Molecular Physiol.) 284:L689-L700.
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